BIO GENETICS LAB two QUESTIONS HELP

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Need Help WITH BLANK BOX QUESTION

(question 1 and iv in decision part. )

* Information technology would be nice if you can check my answeres too.

NOTES:

Tube Sample Lane

one 1 kb Deoxyribonucleic acid ladder ane

2 Female parent'south Deoxyribonucleic acid 2

three Kid's DNA iii

4 A.F. #one Deoxyribonucleic acid 4

5 A.F. #ii Deoxyribonucleic acid 5

Record the distance each ladder band moved from the well in mm along with the size of the DNA fragments in that ring in bp units, based on the bp given in step 19 , in your Lab Notes.

Lane one:

1,000 bp 34 mm

900 bp 36 mm

800 bp 38 mm

700 bp 40 mm

600 bp 43 mm

500 bp 47 mm

400 bp 51 mm

300 bp 56 mm

250 bp lx mm

200 bp 64 mm

150 bp 69 mm

100 bp 77 mm

fifty bp 90 mm

Lane two:

37 mm 850bp

59 mm 265bp

Lane 3:

37 mm 850bp

44 mm 575bp

Lane 4:

41 mm 670bp

43 mm 600bp

Lane v:

44 mm 575bp

55 mm 320bp

Experiment: Agarose Gel Electrophoresis of Deoxyribonucleic acid Fragments

Lab Results

  1. List the distances traveled in mm for the bands in the Dna ladder in the tabular array below.
    Remember, smaller fragments travel farther than longer ones, so the height-nigh band will be the 1,000 bp sized DNA fragments whereas the bottom-most ring will be the fifty bp sized DNA fragments.
    Deoxyribonucleic acid Ladder
    Band Distance (mm)
    50 bp  90 mm
    100 bp  77 mm
    150 bp  69 mm
    200 bp  64 mm
    250 bp  sixty mm
    300 bp  56 mm
    400 bp  51 mm
    500 bp  47 mm
    600 bp 43 mm
    700 bp xl mm
    800 bp 38 mm
    900 bp 36 mm
    ane,000 bp 34 mm
  2. Whose sample had the approximately 570 bp and 320 bp sized DNA fragments?
  3. What were the sizes of the DNA fragments for alleged father #1?

Data Analysis

  1. Which size Deoxyribonucleic acid fragment did the child inherit from her mother?
  2. Which alleged begetter, if any, can be definitively ruled out as the kid's biological begetter?

Conclusions

  1. How are new molecules of DNA synthesized in living cells?
  2. What is the role of Deoxyribonucleic acid?

    Dna has genetic data that controls our cells. So, Dna is like a blueprint that shows how to construct components of cells like proteins and ribonucleic acid (RNA). This information is carried down to newer generations through inheritance.

  3. If each individual has such a small amount of Deoxyribonucleic acid in their cells, how do the bands on the gel contain enough DNA to exist visible?

      In society to make Dna visible. The Gel has to be soaked in a dye (ethidium bromide) to bind with the Deoxyribonucleic acid and rinsed off after. Ethidium bromide helps to brand DNA visible by glowing brightly in UV rays.

  4. Humans only accept a few eye colors and only 4 ABO-based claret types. How tin Dna tests definitively identify individuals when many people take dark-brown eyes or type A claret?
  5. Suppose a suspicious hair was found in a victim'south dwelling. A gel is gear up upward with the DNA fragments of ii suspected criminals in lanes 4 and 5, the Dna fragments of the suspicious hair in lane iii, and the victim'south Dna fragments, as a negative control, are in lane 2. A Deoxyribonucleic acid ladder is in lane ane. The resulting gel is below. Which suspect, if whatever, committed the crime? Explain your answer.

     Lane 4,five (two suspected criniminals)

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Answer( ane )

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